Mascot Search Results

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Email           : 
Search title    : Sequence Query Example (error tolerant tag)
Database        : SwissProt 2014_04 (544996 sequences; 193815432 residues)
Timestamp       : 20 May 2014 at 11:02:18 GMT
Protein hits    : CASB_BOVIN Beta-casein OS=Bos taurus GN=CSN2 PE=1 SV=2
  CLU_DICDI Clustered mitochondria protein homolog OS=Dictyostelium discoideum GN=clua PE=1 SV=2
  PESC_NEUCR Pescadillo homolog OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) GN=nop7 PE=3 SV=1

Mascot Score Histogram

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 33 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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1.    CASB_BOVIN    Mass: 25091    Score: 78     Matches: 1(1)  Sequences: 1(1)
 Beta-casein OS=Bos taurus GN=CSN2 PE=1 SV=2
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Unique  Peptide
1   1031.4000   2060.7854   1980.8548   79.9306 0  78  1.7e-06 1  U    K.FQSEEQQQTEDELQDK.I

 
      Proteins matching the same set of peptides:
      CASB_CAPHI    Mass: 24849    Score: 78     Matches: 1(1)  Sequences: 1(1)
 Beta-casein OS=Capra hircus GN=CSN2 PE=2 SV=1
      CASB_SHEEP    Mass: 24859    Score: 78     Matches: 1(1)  Sequences: 1(1)
 Beta-casein OS=Ovis aries GN=CSN2 PE=1 SV=3

2.    CLU_DICDI    Mass: 148595   Score: 78     Matches: 1(1)  Sequences: 1(1)
 Clustered mitochondria protein homolog OS=Dictyostelium discoideum GN=clua PE=1 SV=2
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Unique  Peptide
 1   1031.4000   2060.7854   1798.8949   261.8906 1  78  1.7e-06 1  U    K.LGGTPEEQQKDIEDLK.A


3.    PESC_NEUCR    Mass: 75247    Score: 78     Matches: 1(1)  Sequences: 1(1)
 Pescadillo homolog OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) GN=nop7 PE=3 SV=1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Unique  Peptide
 1   1031.4000   2060.7854   1596.7380   464.0475 0  78  1.7e-06 1  U    K.AVTNGEEQQQGPDPK.V


Search Parameters

Type of search         : Sequence Query
Enzyme                 : Trypsin
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 0.2 Da
Fragment Mass Tolerance: ± 0.2 Da
Max Missed Cleavages   : 1
Instrument type        : ESI-QUAD-TOF
Number of queries      : 1
Mascot: http://www.matrixscience.com/
Top scoring peptide matches to query 1
etag(977.4,[Q|K][Q|K][Q|K]EE,1619.7)
Score greater than 33 indicates identity
Score    Expect     Delta  Hit  Protein     Peptide
78.1 1.7e-06 79.9306 1 CASB_BOVIN K.FQSEEQQQTEDELQDK.I
78.1 1.7e-06 261.8906 2 CLU_DICDI K.LGGTPEEQQKDIEDLK.A
78.1 1.7e-06 464.0475 3 PESC_NEUCR K.AVTNGEEQQQGPDPK.V